外源性dsRNA 对蛋鸡卵泡CART基因表达及雌激素和孕酮分泌的影响

doi:10.11843/j.issn.0366-6964.2016.03.013

Abstract

Abstract:

To investigate the effect of double-stranded RNA(dsRNA) on cocaine-and amphetamine-regulated transcript(CART) gene expression and estrodiol(E₂) and progestin(P) secretion in follicles of Highland Brown Laying hen.A total of 60 healthy layers at 20-week-old were selected and randomly divided into 3 experimental groups(A group：control group；B group：with treatment of dsRNA intravenous injection every 5 d；and C group：with treatment of dsRNA one initial injection).After 30 d of feeding，the different size of 3-5，6-8，9-12 mm follicles of 10 layers from each group were collected for analyzing mRNA expression of CART and determining the concentration of E₂ and P.The results showed that mRNA expression of CART was decreased in group B and C，compared with the control group.Especially，mRNA expression of CART in 6-8 mm follicle was lower than that in the control group with significant difference（P＜0.05）;The levels of E₂ was increased in the follicles with the size of 6-8 and 9-12 mm in group B with significant difference（P＜0.05）；However，there was no significant difference in the level of P among different treatment groups.The results suggested that intravenous injection of dsRNA could affect the follicular development through inhibiting the mRNA expression of CART and promoting the E₂ production in layer.

CLC Number:

S831.2

LI Peng-fei，MENG Jin-zhu，YU Xue-jing，PANG Yu-ying，DU Hai-yan，LI Xiao-ming，YAO Xiao-lei，ZHAO Miao-miao，Lü Li-hua. Effects of Exogenous dsRNA on CART Gene Expression and Secretion of Estrodiol and Progestin in Layer Follicles[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 515-520.

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Effects of Exogenous dsRNA on CART Gene Expression and Secretion of Estrodiol and Progestin in Layer Follicles

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